There is evidence supporting the influence of experimenter gender on a variety of psychological and physiological variables [28]. 304 Old Main. Temperature regulation specifically affects autonomic and behavioral effectors, including pharmacokinetics and metabolism of drugs and other chemicals. The article was extensively re-written and updated by Dr. Goldi Kozloski in July 2019. This immobile behavior is thought to indicates a state of despair in which the rat has learned that escape is impossible and resigns itself to the experimental conditions. Traditionally mice are initially picked up by the base of the tail, sometimes with a hand positioned to support the animal’s body. To initially restrain a rat, the handler should gently grasp it around the shoulders. The standard protocol to handle laboratory mice is to pick them up from the tail in the middle part of the tail and not from the tip. Tuganbaev T, Mor U, Bashiardes S, Liwinski T, Nobs S, Leshem A. Badimon A, Strasburger H, Ayata P, Chen X, Nair A, Ikegami A. OSVPR Phone: 814-863-9580. Researchers should always follow institutional guidelines for example, the US National Institutes of Health Guide for the Care and Use of Laboratory Animals (8th edition) or ARRIVE [2] or European Communities Council Directive (86/609/EEC) [3], in conducting experiments with animals, and seek approval from local Institutional Animal Care and Use Committee, especially in cases where care and handling of animals deviate from standard practices. The competency level of the personnel who perform the procedures 4. Mice that aren’t used to being handled are easily stressed, which … In a 2012 article titled "Playful handling by caretakers reduces fear of humans in the laboratory rat" [17], the authors tested four scenarios regarding rat handling, including (1) minimal handling; (2) daily exposure to a passive hand for 2 min; (3) daily tickling for 2 min; and (4) daily hand restraint for 2 min (similar to pinning by a dominant rat). Understanding proper laboratory behavior while handling mice. The "cupping" technique may be successfully utilized without fear that the mouse may immediately jump away by first holding the mouse in a closed hand for up to 30 seconds to familiarize the mouse with the handler [21]. The responses were comparable in the light and dark phases of the diurnal cycle and were not dependent on the experience of the handler. Reducing mouse anxiety during handling: effect of experience with handling tunnels. Davis H, Taylor AA, Norris C. Preference for familiar humans by rats. Indeed, stress has been shown to significantly affect heart rate, body temperature, blood glucose and various hormone levels in laboratory mice (for a review, see Balcombe JP et al. The researchers showed that mice of all strains and sex developed a consistent voluntary interaction in the ninth daily handling session among all the three handling methods – the tail, home cage tunnel, or "cupped" on open hand [22]. Used to analyze web traffic to improve the user experience. A summary and overview of the mice and rats used in biomedical research, based on a survey of formal publications. Handling mice is an unavoidable part of an animal caretaker’s routine, and the stress this causes to laboratory mice is an often overlooked factor that may affect experimental outcomes. Picking mice up by the tail is probably the most common method investigators use to catch and transfer mice from one place to another. Guide for the Care and Use of Laboratory Animals. Mice in general are maintained on 12 h light:dark cycles, for example, lights on from 6 am to 6pm [4] or 7 am to 7 pm [5]. Laboratory mice are under stress too, although perhaps not the same kind of stress that their overworked caretakers or grant-writing post-docs are under! The FST is used to measure depressive behaviors in rodents [24]. The way of handling laboratory mice is of critical importance since it can have negative effects on the mice behavior, and induce fear, anxiety responses , and anhedonia - a core symptom of clinical depression which can be measured in rodents by assessing how they consume a sucrose solution . Such trend is already apparent in behavioral studies where familiarity with the animals is described in the material and methods section: "mice were allowed to habituate to different holding rooms for behavioral experiments for two weeks before testing, and to experimenters for at least three days before experiments [27]. J Vis Exp. Office of the Senior Vice President for Research. The importance of laboratory mice in experimental microbiology and biomedical research is indisputable, and their care and handling is a contributing factor to the quality of the science resulting from their use. They also noticed the same response in mice after being restrained by the scruff of the neck. Mice often cost less to purchase and maintain, so they are better suited to the large samples needed for genetic mapping and crossing studies. To daily tickling assessments and mitigation measures are dependent on the purpose the. Tunnel towards the animal without direct contact familiarity with the animal without direct contact mouse! Same response in mice the first ( 1880–1950 ) was when mice rats... Assessments and mitigation measures are dependent on: 1, in one study, exposure of.. Repeats health screening on rodents housed in the laboratory under stress too, although not! 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