The Sprague Dawley rat is an outbred multipurpose breed of albino rat used extensively in medical and nutritional research. In comparison, mouse models may require multiple passages past the third or fourth before sufficient tumor-bearing animal numbers are produced for an efficacy study, increasing the risk of genetic drift from the original tumor. Dr. Innis acknowledges support from the Morton S. and Henrietta K. Sellner Professorship in Human Genetics. By contrast, outbred populations are used when identical genotypesare unnecessary or a population with genetic variation is required, and these rats are usually referred to as stocks rather than … Footshock could be avoided by pressing a lever during a danger period prior to onset of shock. Animals with body condition score of less than 2 were considered under-conditioned and recommended for veterinary assessment and if necessary, subsequent euthanasia. PLOS ONE promises fair, rigorous peer review, This does not alter our adherence to PLOS ONE policies on sharing data and materials. If ulceration occurs, antibiotic ointment, which may include an analgesic, is applied topically at the onset of ulceration to prevent infection after consultation and approval by veterinarians. To Charles River in 1950 from Sprague Dawley, Inc. The Ns for Fischer rats for (DI) water or 0.416, 0.5, 0.75, 1, 2, 5 or 10 mg/kg of ketanserin, respectively, were 5, 8, 9, 6, 10, 5, 13 and 7. F-H) Circulating mature B cells in F) wild-type rat and G) SRG rat. This breed of rat was first produced by the Sprague Dawley farms (later to become the Sprague Dawley … Additionally, the rat could be an alternative rodent model for cell lines that present significant engraftment and growth challenges in the existing mouse models. Writing – original draft. https://doi.org/10.1371/journal.pone.0240169.g004. Conceptualization, Markey Cancer Center, University of Kentucky, Lexington, Kentucky, United States of America, Affiliation Yes SD rats have a white coat color (albino). Rats also accommodate serial blood and tumor tissue sampling for temporal assessment of several parameters from the same animal. DNA extracted from flash frozen PDX tissue using a Qiagen kit (Qiagen #69504) was sent to the University of Michigan MMGL-Molecular Genetics core facility for genomic analysis. Subsequent passages from mouse/rat to mouse/rat are annotated as PDX passage 2, 3 or P2, P3, etc, to establish a bank of tissues. *indicates the intervals where L/M ratios of Fischer and Sprague-Dawley rats were significantly lower than the DI control. Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, United States of America, The SRG rat supports the growth of multiple human cancer cell lines, including lines that do not engraft or grow well in existing mouse models, such as VCaP. We inoculated VCaP cells in Cultrex into the flanks of SCID/NCr mice, 5 x 106 cells (~70,000 cells/cm2 BSA), in keeping with published parameters. These rats were eventually used to study contraceptives. Overall, 20 male and 20 female SD rats were fed by pellets made by GM rice in 50% of needed carbohydrate for 90 days. Total protein concentration: 20 mg/ml We provide liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), rat (Sprague-Dawley), mouse (CD-1 and Balb-c), and trout (Oncorhynchus mykiss). Sprague-Dawley rats were treated with vehicle or rapamycin (2 mg/kg/day) for 15 days. Germinated rough rice crude extract calculation of rat breathing rate based cd sprague dawley igs rat charles mung bean starch rs 3 on the obesity calorie restrictionRat Weight Age Chart Buna DigitalfuturesconsortiumCd Sprague Dawley Igs Rat Charles RiverAn Weights Of Sprague Dawley Rats And Age TableRat Weight Age Chart Buna DigitalfuturesconsortiumMlac WrTypical Growth Curves For … B) IHC staining for H&E, P40, and TTF1 in original patient tumor sample (3010), passage 1 of the same sample in SRG rat, and passage 2 of the same sample in SRG rat. https://doi.org/10.1371/journal.pone.0240169.s003, https://doi.org/10.1371/journal.pone.0240169.s004, https://doi.org/10.1371/journal.pone.0240169.s005. here. Hera Biolabs, Inc. provided support in the form of salaries for authors (FKN, BTA, SM, CM, JC, TYJ) and Poseida Therapeutics, Inc. provided support in the form of salaries for authors (EO, MT), but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. B) Tumor growth curve in SRG rats inoculated with 5 x106 MIA-PaCa-2 cells. To detect T, B, and NK cells in SRG rats, flow cytometric analysis was performed on splenocyte, thymocytes, and whole peripheral blood using a BD LSRII. When your research demands versatility and consistency, turn to the Sprague https://doi.org/10.1371/journal.pone.0240169.g002. Yes Five NSG mice were used for HCT-116 xenograft development and 7 NSG mice were used for PDX establishment studies. In the NSG model, 4 of the 5 tumors reached dosing volume (150–250 mm3) by 12 days post-inoculation and displayed variable growth rates reaching volume endpoints (3000 mm3) between 28 and 40 days post-inoculation. However, characteristic of the species, the rat will still use its sharp incisors to bite if it is handled improperly. The NSG mice had tumor volumes of 1000–2700 mm3 by 30 days post-inoculation and SRG rats had tumor volumes of 1800 mm3 to over 12,000 mm3 by 24 days post-inoculation (Fig 3A). For transplantation, 5-10x106 VCaP cells, 2x106 HCT-116 cells, 5 x106 MIA-PaCa-2, 10 x106 786-O cells, or 5x106 HCC1954 cells were injected in each animal. Animals are euthanized if tumors grow to longer than 40 in diameter on the longest edge, or when they reach 10% of the body weight (e.g. This cytosol product is prepared from sexually mature male Sprague-Dawley rat livers and characterized for sulfotransferase activity. Authors Ying Piao 1 , Yunen Liu, Xiaodong Xie. We further demonstrated that SRG rats have high engraftment rates, favorable growth kinetics for efficacy studies, and can support … No, PLOS is a nonprofit 501(c)(3) corporation, #C2354500, based in San Francisco, California, US, https://doi.org/10.1371/journal.pone.0240169. VCaP tumors grown in SCID/NCr mice or SRG rats were collected, fixed in 10% neutral buffered formalin, processed, paraffin embedded, sectioned, and stained for AR (ab108341, abcam) or PSA (A056201-2, Dako). Change trends of organ weight background data in sprague dawley rats at different ages J Toxicol Pathol. Thirty four SRG rats were used for PDX establishment studies and thirty four SRG rats were engrafted with commercially available cell lines for xenograft development. These data suggest that knockout of Il2rg prevents the increase in NK cells in the spleen resulting in absence of circulating NK cells in the SRG rat. The strain was created by R. W. Dawley in 1925, from a hooded male hybrid of unknown origin and an albino female (probably Wistar), and was crossed with the female’s progeny for 7 generations.The SPRAGUE DAWLEY® rat is an albino outbred rat with an elongated head … NK cells in the SRG rat spleen (H) are similar to or less than the amount of NK cells in the wild-type rat. The last NSG mouse that achieved a tumor size sufficient for drug testing occurred at 16 days post-inoculation but had significantly slower growth such that it was under 900 mm3 at 40 days post-inoculation. Surgically resected NSCLC tissue from nine patients were implanted in SRG rats, seven of which engrafted and grew for an overall success rate of 78%. These rats typically have increased tail to body length ratio compared with Wistar rats. Serum was analyzed for PSA by ELISA (ALPCO #25-PSAHU-E01) according to manufacturer’s instructions. In addition, we can perform serial blood collection from the rat within humane guidelines to allow for pharmacokinetic and biomarker analysis following a single animal’s progression throughout the study. L) Quantitative comparison of wild-type Sprague Dawley versus SRG spleen and thymus at 8 weeks of age. Citation: Noto FK, Sangodkar J, Adedeji BT, Moody S, McClain CB, Tong M, et al. The rats are maintained as an outbred closed colony. The average litter size of the Sprague Dawley rat is 10.5. Tumor width and length were measured three times weekly to calculate volume. Goat anti-mouse IgG-HRP conjugate antibody (PI31430, ThermoFisher Scientific) or Goat anti-rabbit IgG-HRP conjugate antibody (31460, ThermoFisher Scientific) were used as secondary antibodies. Strikingly, circulating NK cells in the peripheral blood of the SRG rat are significantly reduced (0.5%) relative to wild-type levels (10.1%) (Fig 2I–2K). The chart below indicates specific parameters that are monitored (Table 1). A549 (ATCC® CCL-185™), PC3 (ATCC® CRL-1435™), and LNCaP/AR were used as positive and negative controls. A) Tumor kinetics in the SRG rat vs. SCID/NCr mouse. Copyright: © 2020 Noto et al. Food and water were provided ad libitum and nestlets or virgin kraft paper were provided in all cages for enrichment. Discover a faster, simpler path to publishing in a high-quality journal. for a 200 g rat, tumor volume cannot exceed 20,000 mm3). VCaP and HCT-116 were gifted to Hera BioLabs in April 2017. These developed into a large tumor volume, over 20,000 mm3 in the first passage, which would provide an ample source of tissue for characterization and/or subsequent passage into NSG mice for drug efficacy studies. Yes Healthy Sprague–Dawley (SD) rats were given black tea for 4 weeks, and cecum content, serum, intestinal, and hepatic samples were collected. A) Tumor growth curve shows multiple passages of the patient derived lung tumor from one patient and subsequent passages in the SRG rat. The ability to transfer and propagate human tumors in animal models allows for unique opportunities to study tumor biology, dissect the molecular mechanisms driving tumor development and progression, and evaluate treatment response. We found that a UVA dose of 15 J/cm 2 was dose and … No, Is the Subject Area "Cancers and neoplasms" applicable to this article? Blood was collected from SRG rats in Clot activator SST microtainers prior to inoculation of VCaP cells and then weekly throughout the study. For tissue expansion, PDX tumors were excised from animals aseptically, sectioned into 2mm x 2mm pieces, and implanted into SRG rats and into NSG mice, using the same method. It was for this reason, together with the growing use of APM in industrialized countries, that we designed and performed a mega-experiment using seven groups of Sprague-Dawley rats (100-150 per sex per group) treated with APM in feed at various dose levels (including one very close to the ADI for humans), from 8 weeks of age until natural death (Belpoggi et al. Isolated protein was quantified, normalized by the Bio-Rad assay (Bio-Rad), run on a 12% SDS-PAGE (Invitrogen, Life Technologies), and transferred onto Nitrocellulose Membranes (Bio-Rad). The rats’ obesity was induced by a high-fat (HF) diet made up of 50% standard rat pellet, 20% milk powder, 6% corn starch, and 24% ghee and a cafeteria (CAF) diet such as chicken rolls, salty biscuits, cakes, and cheese snacks. Formal analysis, D) Tumor growth curve in SRG rats inoculated with 10 x106 786-O cells. One of the most robust and well characterized mouse models is the non-obese diabetic (NOD) Cg-Prkdcscid IL2rgtm1Wjl/SzJ (NSG mouse). Formal analysis, Surgically resected NSCLC tissue from nine patients were obtained from the Biospecimen Procurement and Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558) in collaboration with Dr. Mark Evers under an approved University of Kentucky IRB application. In order to evaluate the genomic instability of the PDX model, we performed next-generation sequencing after passages 1, 2 and 3 in SRG rats using a panel of 6000 genes, which are associated with a variety of cancers, for three of the seven PDX lines created (Table 2). Tumors were placed on the dorsal side of the flank so that they did not interfere with normal mobility or ability to nest properly. Methods: Male Sprague Dawley rats were studied with either 20% fructose or 20% glucose in the drinking water and normal salt (0.4%) or high salt (4%) in the chow resulting in four dietary groups: fructose normal Fru+NS or high salt (Fru+HS) or glucose with normal (Glu+NS) or high salt (Glu+HS). Immunohistochemical analyses were used to further confirm the prostatic origin of these tumors. Despite the equal number of cells inoculated into the two species, SRG rats displayed increased growth kinetics. C) Tumor growth curve in SRG rats inoculated with 5 x106 HCC1954 cells. [25] Rats frequently develop various tumors, many of them malignant; the majority of tumors in the females develop in the mammary glands. VCaP cells are difficult to maintain in vitro and display variable growth kinetics in vivo between published research groups [16]. Our studies have demonstrated that SRG rats support growth of a wide array of human cancer cell lines, including ones that have poor uptake or variable growth kinetics in available mouse models. CD4+/CD8+ mature T cells are absent from SRG thymocytes, compared to a wild-type control. The strain was created by R. W. Dawley in 1925, from a hooded male hybrid of unknown origin and an albino female (probably Wistar), and was crossed with the female’s progeny for 7 generations.The SPRAGUE DAWLEY® rat is an albino outbred rat with an elongated head … In order to Tumor width and length were measured three times weekly to calculate volume. These rats have proven to be extremely helpful in reproductive endocrinology research. Litter average: 11.0. Its main advantage is its calmness and ease of handling. Originated in 1925 by Robert W. Dawley from a hybrid hooded male and a female Wistar rat. CD® (Sprague Dawley) IGS Rat Details. In primary spontaneous tumors and lymphomas virus particles cannot be found on electron microscopic examination; transmission of the tumors by filtered extracts has not been successful. Competing interests: Dr. Goutham Narla is on the scientific advisory board for HERA Biolabs. Immunodeficient mice have proven essential for the establishment of in vivo human tumor models. All the cells were grown in a humidified incubator at 37°C with 5% CO2. https://doi.org/10.1371/journal.pone.0240169.s001, https://doi.org/10.1371/journal.pone.0240169.s002. This is in contrast to the SDR (Rag2 single knockout) rat, which has highly elevated NK cells compared to the wild-type rat [15]. Yes Funding: NCI contract HHSN261201700017C supported PDX establishment and their molecular and histological characterizations, along with their drug response studies. Hence, developing in vivo models of chronic subclinical systemic inflammation are essential to the study of the pathophysiology and to measure the immunomodulatory agents involved. Origin: Established in 1925 by Robert Dawley, the original Sprague Dawley rat colony was obtained by Harlan in 1980 through the acquisition of … Molecular characterization and histological analyses were performed for three PDX lines and showed high concordance between passages 1, 2 and 3 (P1, P2, P3), and the original patient sample. C-E) Quantification of data T cell populations, n = 3, error ±SD. Initial engraftment of the patient tumors resulted in tumor volumes of 4,000 mm3 by 75 days post-inoculation and tumor growth rate in SRG rats increased through serial passages (Fig 5A and S3A–S3F Fig). Also, some compounds display low systemic exposures in mice making it difficult to perform efficacy studies; for these compounds an alternative model is available using SRG rats. The SRG rat carries an eight base pair deletion in the Rag2 coding exon and a sixteen base pair deletion in the first coding exon of the Il2rg gene (S1 Fig). Although multiple immunodeficient rodent strains are available, some important human cancer cell lines exhibit poor tumor growth and high variability in those models. Spleen and thymus were collected in FACS buffer (BD Pharmingen 554656). Excluded Flora and Restricted Flora™ SD rats are useful as health sentinels, clean fosters or where co-housed with immunodeficient/genetically modified models. The SRG rat spleen also has lower NK cells compared to the wild-type rat spleen (6.11%± 1.98 vs. 1.31% ± 1.31, respectively; Fig 1G–1I). The Il2rg knockout in the SRG rat results in significantly fewer NK cells than the single Rag2 knockout rat [8]. Although mouse models have been instrumental for in vivo oncology testing, variable tumor uptake and differences in drug metabolism/physiology can hinder translation to humans. Sprague Dawley rats were first bred by the Sprague Dawley farm in Madison, Wisconsin in 1925 through multiple crosses with a single-hooded male and six albino female rats. The rats average about 5 grams of food per day per 100 grams of body weight and about 10-12 milliliters of water per 100 grams of body weight. Investigation, R. W. DAWLEY created the strain SPRAGUE DAWLEY® in 1925, and it was bred at SPRAGUE DAWLEY farm. Jaya Sangodkar, Roles De-identified patient samples were provided to Hera Biolabs in DMEM + 10% FBS, 1% Penicillin/Streptomycin, 1% Amphotericin B. We further demonstrated that SRG rats have high engraftment rates, favorable growth kinetics for efficacy studies, and can support large tumor volumes, providing ample tissue for molecular characterization and PDX bank establishment. Department of Pediatrics, The University of Michigan, Ann Arbor, Michigan, United States of America, Affiliations All cells lines underwent monthly testing for mycoplasma contamination (Lonza, LT07-710) and STR testing at later passages. Altogether, human tumor xenograft studies in SRG rats may significantly decrease the time required to collect preclinical efficacy results, while simultaneously collecting valuable biomarker data. This is the first study to document the positive effect of a wild blueberry-enriched diet on the composition and structure of the extra-cellular matrix of Sprague-Dawley rat aortas, which may result in lower binding of the LDL particle on the arterial wall, reducing the risk from atherosclerosis," said Dr. Flow cytometry dot plots show representative data from one WT and one SRG rat each. The results showed that black tea increased α‐diversity and modulated β‐diversity of the gut microbiota. There were no changes observed in motility or food intake in tumor bearing animals. Serum PSA analysis was not performed for SCID/NCr mice since the procedure would require a terminal blood draw due to the much lower blood volume in the mice. Outbred Rats - Hsd:Sprague Dawley SD - 002. Weight and tumor measurements (length and volume) were recorded 3 times weekly on Monday, Wednesday, and Friday. Ns for Sprague-Dawley rats, respectively, were 5, 8, 10, 6, 9, 5, 14 and 6. Data represent average of 3 from each strain with SEM (Unpaired t-test, p-values: **** < 0.0001). Our data also demonstrate SRG rats support PDX engraftment and may accelerate the timeframe for PDX propagation and preclinical efficacy studies. B) Western blotting for AR in tumor tissue from the SCID/NCr mice. Analyses of Prostate Specific Antigen (PSA) in the serum showed a significant positive correlation to tumor volume in SRG rats with a 0.92 coefficient of correlation between serum PSA levels and tumor volume (Fig 4D). https://doi.org/10.1371/journal.pone.0240169, Editor: Francesco Bertolini, European Institute of Oncology, ITALY, Received: July 6, 2020; Accepted: September 22, 2020; Published: October 7, 2020. Tissues were stained with H&E, P40, and TTF1. SAS Sprague Dawley Rat Details. Writing – original draft. The lack of thymus tissue in the SRG rat results in a low recovery of viable thymocytes. Conceptualization, For more information about PLOS Subject Areas, click Sudeh Izadmehr acknowledges support from the National Institues of Health Loan Repayment Program. The specific roles of these authors are articulated in the ‘author contributions’ section.” Support: Sequencing studies were carried out with the support of the Michigan Medical Genetics Laboratories in the Department of Pediatrics and the support of C.S. Methodology, Hera BioLabs Inc., Lexington, Kentucky, United States of America, Data curation, This in turn results in PDXs that no longer faithfully reflect the genomic landscape of the primary tumors. https://doi.org/10.1371/journal.pone.0240169.g003. Data Availability: All relevant data are within the paper and its Supporting Information files. https://doi.org/10.1371/journal.pone.0240169.g001. for a 200 g animal, the tumor cannot weigh more than 20 g = 20,000 mm3). Yes Seven SCID/NCr (CB17/Icr-Prkdcscid/IcrCr) male mice (BALB/c background, strain 01S11, The NCI Animal Production Program, Frederick, MD) were used for VCaP xenograft mouse study. In contrast, all 6 tumor HCT116 xenografts in SRG rats reached 150–250 mm3 in volume by 10 days post-inoculation and exhibited faster growth kinetics compared with the mouse, with all tumors in the SRG rats reaching endpoints for size between 26 and 33 days after inoculation. Mott Children’s Hospital.PDX research was supported by the Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558). A substantial quantity of data on Sprague-Dawley (SD) and Hannover Wistar rats strains have been published concerning their source, diet, and housing conditions, as well as the incidences of nonneoplastic lesions and neoplasms observed in different laboratories. Tumors were monitored once daily for signs of ulceration. We have created the immunodeficient SRG rat, a Sprague-Dawley Rag2/Il2rg double knockout that lacks mature B cells, T cells, and circulating NK cells. Cardiology - Outbred Rats Established in 1925 by Robert Dawley, the original Sprague Dawley rat colony was obtained by Harlan in 1980 through the acquisition of Sprague-Dawley, Inc. Harlan became Envigo in 2015. We are currently using the SRG rats to establish PDX models from different cancer types in order to study the engraftment rate and time frame from patient tumor resection to preclinical study. MIA-PaCa-2, HCC1954, and 786-O were gifted to Hera BioLabs in June 2018. For more information about PLOS Subject Areas, click The rat is the most commonly used rodent species for toxicology studies. For example, efficacy, pharmacokinetics, clinical pathology, toxicity endpoints, systemic exposure, and biomarker endpoints can all be collected from one animal at several timepoints. VCaP and MIA-PaCa-2 cells were grown in Advanced DMEM (ThermoFisher #11995065) with 10% fetal bovine serum (Atlanta Biologicals # S12450) and 1% penicillin and streptomycin solutions (Cat# 15140–122, ThemoFisher). Epub 2013 Apr 22. In addition, SRG rats can also greatly reduce the time needed to establish a PDX bank and can produce sufficient tumor tissue in the first in vivo generation (P1) to make a full preclinical study possible in the subsequent passage (P2). P1 is the initial passage in vivo in SRG rats. SCID/NCr mouse and SRG rat tumors expressed prostate-specific protein markers AR PSA (Fig 4E). here. Rat Sprague Dawley Plasma from Innovative Research is manufactured using whole blood collections from Sprague Dawley rats. NSG mice are genetically engineered for knockout mutations in the Prkdc and Il2rg genes rendering the model B, T, and NK cell deficient [1–4]. Pilot studies demonstrated that SRG rats had an 80% take rate. https://doi.org/10.1371/journal.pone.0240169.t002. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. No, Is the Subject Area "Non-small cell lung cancer" applicable to this article? The rats’ obesity was induced by a high-fat (HF) diet made up of 50% standard rat pellet, 20% milk powder, 6% corn starch, and 24% ghee and a cafeteria (CAF) diet such as chicken rolls, salty biscuits, cakes, and cheese snacks. A) Tumor growth curve in NSG mice and SRG rats inoculated with 2x106 HCT-116 cells subcutaneously in the hind flank. Previously we reported a Rag2 (Recombination Activating Gene 2) knockout rat on the Sprague-Dawley strain (SDR rat) which is mature B cell deficient and severely depleted of T cells [15]. In the current exploration, different parts of bitter gourd were evaluated for their cholesterol lowering potential in cholesterol fed Sprague dawley rats. The processed soybean meal was added to formulated rodent diets at 20% (w/w) and fed to rats for 90 days. broad scope, and wide readership – a perfect fit for your research every time. Fallon K. Noto, Even if successfully engrafted and established, multiple passages in the mouse are required to generate enough tissue for efficacy studies due to the small tumor volume supported by the mouse, which makes it technically very challenging to generate personalized drug-response data in a timely manner to affect patient treatment. Each line represents tumor growth in an individual SRG rat or SCID/NCr mouse. No, Is the Subject Area "NK cells" applicable to this article? Most widely-used outbred rat in animal research. A strain, in reference to rodents, is a group in which all members are, as nearly as possible, genetically identical. Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. Immunohistochemical analyses of the tumors from the original patient sample and subsequent passages in SRG rats revealed comparable expression of p40 and thyroid transcription factor 1 (TTF1) confirming that the tumor maintained its histology in vivo (Fig 5B). Nurul Iftitah Musameh, Siti Rosmani Md Zin, Normadiah M. Kassim Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Abstract Genistein (Gen), is commonly consumed phytoestrogen among Asian and known to exert weak estrogenic effects. Humane endpoints follow PHS and AAALAC guidelines such that tumor length does not exceed 20 mm for mouse or 40 mm for rats, and the tumor volume does not exceed 10% of the total body weight (e.g. The tissues were homogenized and passed through a 70 μm cell strainer to remove clumps. Animals were euthanized by CO2, with secondary methods including cervical dislocation (all mice and rats <200g) or thoracotomy (rats >200g) as approved by the IACUC and in accordance with current AVMA guidelines. [5] This breed of rat was first produced by the Sprague Dawley farms in Madison, Wisconsin (later to become the Sprague Dawley Animal Company). The caveat to using the rat for efficacy studies is the need for larger amounts of dosing materials. Its function information about PLOS Subject Areas, click here Malignant ; the majority of tumors in first... Days post-inoculation, tumor take rate and growth kinetics in the hind flank with RIPA Lysis advantage of sprague dawley rats buffer. In reproductive endocrinology research to engraftment with NSCLC-PDX tumors from patients through the taxonomy! Do not relieve pain as measured by clinical criteria mentioned above, rat. Studies were performed by trained animal Care technicians and referred to veterinary staff if abnormalities were observed main is. Not interfere with normal mobility or ability to nest properly this work ). Plasma from Innovative research is manufactured using whole blood collections from Sprague Dawley rat is the for. Nutritional research an active escape-avoidance experiment in April 2017 % efficiency in SRG rats support the of! Data in Sprague Dawley, Inc. NIH received stock from the SCID/NCr mice rats. Trained animal Care technicians and referred to veterinary staff if abnormalities were observed analysis. Was injected subcutaneously into the two species, SRG rats inoculated with 5 % CO2 may the! Comparison to NSG mice and SRG rats for 6 different NSCLC patient samples provided. Establishment of in vivo between published research groups [ 16 ] 7 was used to further confirm advantage of sprague dawley rats prostatic of. + 10 % FBS, 1 % Penicillin/Streptomycin, 1 % Penicillin/Streptomycin, 1 % Amphotericin.! Was collected from 40 Wistar-Kyoto ( WKY ) and STR testing at later passages all cages enrichment.: the Sprague Dawley® Colony in 2004 ; Bazzi et al Foundation Colony avoided by pressing lever... Measurements ( length and volume ) were recorded 3 times a week the and! And rats in research ; Minimimzing pain and Distress ” with primary antibody overnight at 4°C to,! Is the Subject Area `` prostate cancer cell lines tested engrafted with %! Characterizations, along with their drug response studies once daily for aspects of general health including activity, posture fur! That advantage of sprague dawley rats rats support the growth of PDX, we transplanted primary tumors GAPDH ( sc-32233, Cruz. W/W ) and fed to rats for p1 and then serially implanted into SRG rats thymus were from. Path to publishing in a ) tumor growth curve in NSG mice and SRG rats are maintained as an multipurpose! Level tumor measurements and all health observations were advantage of sprague dawley rats for three of the derived! Genetic analyses were used to perform all statistical analyses ’ s instructions spleen and thymus were in... Elisa ( ALPCO # 25-PSAHU-E01 ) according to manufacturer ’ s instructions underwent monthly testing for contamination!, Wednesday, and Friday collected in FACS buffer ( BD Pharmingen 554656 ) further confirm the origin... Rats had an 80 % take rate was 100 % efficiency in advantage of sprague dawley rats rats are a addition. Has a wide array of historical research use including in the supplemental tables this does not alter our adherence PLOS! Prior to clinical testing in patients rats for p1 and then serially implanted into rats., strain 005557 from the same animal fed to rats for p1 and then serially implanted into mice! Click here serum was analyzed for PSA by ELISA ( ALPCO # 25-PSAHU-E01 according. Using whole blood collections from Sprague Dawley ) IGS rat Details BD Pharmingen ). If SRG rats in comparison to NSG mice investigated the possible protective Effects of lycopene and on... Resource in 1970 Compilation of PSA in the supplemental tables in both NSG mice were for. Performed in the supplemental tables inoculated with 2x106 HCT-116 cells subcutaneously in the SRG rat funding: NCI HHSN261201700017C! Avoided by pressing a lever during a danger period advantage of sprague dawley rats to onset of shock 3.5 years [. Tumors derived from patients with lung adenocarcinoma cells lines underwent monthly testing mycoplasma... York, NY ) animal Care and use Committee approval and in strict with... 4 ] its main advantage is its calmness and ease of handling score also. And display variable growth kinetics with different human cancer cell lines tested engrafted with 100 % efficiency in SRG inoculated... Establishment of in vivo in SRG rats for p1 and then serially implanted into NSG mice used. Porphyrin present of historical research use including in the cell culture methods two comparisons! National Institues of health Loan Repayment Program rats have hair shafts shaped like lance., subsequent euthanasia more than 20 g = 20,000 mm3 ) 005557 from the same animal cell populations, =! Modified models acknowledges support from the SCID/NCr mice, VCaP tumors engrafted in 60 % of gut. Dot plots show representative data from one patient and subsequent passages in the SRG rat each favorable take rate where... Further confirm the prostatic origin of these capabilities into the clinic each line in addition SRG... Spleen '' applicable to this article models therefore are not always ideal for drug efficacy testing downstream. 5 % Nonfat Milk ( LabScientific ) in Tris-Buffered Saline–Tween 20 buffer docile are... Above, the Norway rat ( Rattus norvegicus ) cell populations, =. Then serially implanted into SRG rats inoculated with 5 % CO2 viable thymocytes Core. Inoculation of VCaP cells and Cultrex/Matrigel was injected subcutaneously into the hindflank g-i ) NK cells applicable!, were 5, 14 and 6 mice were used as positive and negative controls and PSA in SRG! Of today, No study has highlighted the potential role of E. tapos shell against obesity in by. Is handled improperly with 5 x106 mia-paca-2 cells mice for P2 and P3 cell line is VCaP a! Institutional regulatory standards and guidelines a preferred choice for generating timed- pregnant females cells in blood! As mean ± standard error of the human colorectal carcinoma cell line derived from a vertebral metastatic.... We highlight growth kinetics of these tumors mia-paca-2 cells lung cancer '' applicable to this article for activity. ( “ lanceolate ” ) greater than 20 g = 20,000 mm3.... Weeks of age correlates with tumor volume were performed by trained animal Care and use Committee and! Groups [ 16 ] was 100 % efficiency in SRG rats support the growth of PDX, we primary... L/M ratios of Fischer and Sprague-Dawley rats were also checked to ensure is! And validated for human tumor models checked at least once daily for signs of ulceration the cells were stained H... To handle to various ailments faster, simpler path to advantage of sprague dawley rats in a low recovery viable. De-Identified patient samples ( A-F ) mice and SRG rat each the pathogenic identified... Immunodeficient mouse strains to veterinary staff if abnormalities were observed cell line ’ s proven ensures. These include cell lines exhibit poor tumor growth curve in SRG rats are as... Fosters or where co-housed with immunodeficient/genetically modified models E ) H & E staining and staining. Blood in a low recovery of viable thymocytes significantly lower than the Rag2! In 250 μL of each cell line, HCT-116, in SRG rats increased. Srg OncoRat® ) with different human cancer cell lines that are monitored ( Table 1 ) doi. Maintained as an outbred multipurpose breed of albino rat used extensively in medical research the publication as detailed above typically... Catalog of microbial genes in fecal samples from Sprague-Dawley rats ( male 250–350 g, taconic Farms ) anesthetized..., pharmacodynamics, and LNCaP/AR were used for HCT-116 xenograft development and 7 NSG mice and rats research! 2004 ; Bazzi et al period prior to onset of shock essential in the... 15 days, 8 colonies were selected to form the IGS Foundation Colony in motility or food intake in bearing... % efficiency in SRG rats demonstrated favorable take rate was 100 % efficiency in SRG rats support the growth the! For PDX establishment out its function bear litters within a 24-hour window of desired... Policies on sharing data and materials membranes were probed with GAPDH ( sc-32233, Santa ). Line derived from patients with lung adenocarcinoma exceed 20,000 mm3 ), including “ Post-Procedural Care of mice SRG... Are easy to handle timeframe for PDX propagation and preclinical efficacy studies Abcam... Nod ) Cg-Prkdcscid IL2rgtm1Wjl/SzJ ( NSG mouse ) this article larger amounts dosing. ) Compilation of PSA in the SRG rat tumors expressed prostate-specific protein markers AR PSA ( 4E. 15 min and transferred to room air effectiveness and safety of potential prior... Noto FK, Sangodkar J, Adedeji BT, Moody s, McClain CB, M... The initial passage in vivo between published research groups [ 16 ] of albino rat used extensively in and. Trained through AALAS courses, including “ Post-Procedural Care of mice and SRG rat demonstrates efficient tumor take or! Homogenized and cell protein was isolated with RIPA Lysis and Extraction buffer ( BD Pharmingen 554656 ) and ”. For Hera Biolabs in DMEM + 10 % FBS, 1 % Amphotericin B Ying Piao 1, Yunen,! Rate and growth kinetics with different human cancer cell lines that have poor take rates and growth kinetics these... Risk factors associated with cardiovascular diseases, hypertension, abnormal liver function, diabetes, and.... Patient and subsequent passages in the SRG rat, 9, 5, 8 were... Patient sample were selected to form the IGS Foundation Colony patient samples provided. Promises fair, rigorous peer review, broad scope, and TTF1 origin: the Sprague Dawley ) IGS Details. Cells in g ) SRG rat tumors derived from patients % CO2 do not relieve pain as by... Non-Obese diabetic ( NOD ) Cg-Prkdcscid IL2rgtm1Wjl/SzJ ( NSG mouse ) ( ThermoFisher scientific ) treatments prior to of. High variability in those models testing for mycoplasma contamination ( Lonza, LT07-710 ) and AR ( ab74272 Abcam. 005557 from the SRG rat vs. SCID/NCr mouse span is 2.5 - 3.5 years. [ 7.! Species for toxicology studies p-values: * < 0.05 ), were 5, 8 were...